Linked Life Data

7886686

Source:http://linkedlifedata.com/resource/pubmed/id/7886686

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-4-10
pubmed:abstractText
Murine assays such as the mouse ear swelling test (MEST) and the local lymph node assay (LLNA) are popular alternatives to guinea pig models for the identification of contact sensitizers, yet there has been concern over the effectiveness of these assays to detect weak and moderate sensitizers. Much work has been done to improve the sensitivity of the MEST, including the addition of a vitamin A acetate (VAA) enriched diet, which increases its sensitivity. Vitamin A acetate has been reported to increase the numbers of Langerhans cells (antigen presenting cells) in the skin, which could in turn enhance the cellular immune response. Because the LLNA relies on tritiated-thymidine incorporation by proliferating T cells during the induction phase, we have studied the potential of the VAA diet to enhance sensitivity of the LLNA. Results indicate that the VAA enriched diet significantly increased the LLNA sensitivity to formalin, eugenol, glutaraldehyde, trimellitic anhydride, and an azo dye at concentrations where no proliferation was observed in mice maintained on the standard diet. Maintenance on a VAA diet for 3 weeks prior to initiating the sensitization procedure was optimal. Thus, incorporation of a VAA diet improves the sensitivity of the LLNA as a quick, objective, and relatively inexpensive screen for detecting moderate and weak contact sensitizers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0300-483X
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Dietary vitamin A enhances sensitivity of the local lymph node assay.
pubmed:affiliation
ManTech Environmental Technology, Inc., Research Triangle Park, NC 27709.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.