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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-4-7
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA encoding a zinc transporter (ZnT-1) was isolated from a rat kidney cDNA expression library by complementation of a mutated, zinc-sensitive BHK cell line. This cDNA was used to isolate the homologous mouse ZnT-1 gene. The proteins predicted for these transporters contain six membrane-spanning domains, a large intracellular loop and a C-terminal tail. ZnT-1 is homologous to zinc and cobalt resistance genes of yeast. Immunocytochemistry with an antibody to a myc epitope added to the C-terminus of ZnT-1 revealed localization to the plasma membrane. Transformation of normal cells with a mutant ZnT-1 lacking the first membrane-spanning domain conferred zinc sensitivity on wild-type cells, suggesting that ZnT-1 functions as a multimer. Deletion of the first two membrane-spanning domains resulted in a non-functional molecule, whereas deletion of the C-terminal tail produced a toxic phenotype. Mutant cells have a slightly higher steady-state level of intracellular zinc and high basal expression of a zinc-dependent reporter gene compared with normal cells. Mutant cells have a lower turnover of 65Zn compared with normal cells or mutant cells transformed with ZnT-1. We propose that ZnT-1 transports zinc out of cells and that its absence accounts for the increased sensitivity of mutant cells to zinc toxicity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-1375058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-1508175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-1560036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-1639868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-1653172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-1837150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-2693940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-2806412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-2959555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-3527054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-3649277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-3838360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-3885271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-4058587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-708788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-7919780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8026472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8058041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8065932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8089141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8108390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8172914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8242750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8303295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8419966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7882967-8467794
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
639-49
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7882967-Amino Acid Sequence,
pubmed-meshheading:7882967-Animals,
pubmed-meshheading:7882967-Biological Transport,
pubmed-meshheading:7882967-Cation Transport Proteins,
pubmed-meshheading:7882967-Cell Compartmentation,
pubmed-meshheading:7882967-Cell Line,
pubmed-meshheading:7882967-Cloning, Molecular,
pubmed-meshheading:7882967-Cricetinae,
pubmed-meshheading:7882967-Gene Expression,
pubmed-meshheading:7882967-Genes,
pubmed-meshheading:7882967-Kidney,
pubmed-meshheading:7882967-Membrane Proteins,
pubmed-meshheading:7882967-Mice,
pubmed-meshheading:7882967-Molecular Sequence Data,
pubmed-meshheading:7882967-RNA, Messenger,
pubmed-meshheading:7882967-Rats,
pubmed-meshheading:7882967-Sequence Alignment,
pubmed-meshheading:7882967-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7882967-Structure-Activity Relationship,
pubmed-meshheading:7882967-Transfection,
pubmed-meshheading:7882967-Zinc
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pubmed:year |
1995
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pubmed:articleTitle |
Cloning and functional characterization of a mammalian zinc transporter that confers resistance to zinc.
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pubmed:affiliation |
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
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pubmed:publicationType |
Journal Article,
Comparative Study
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