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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1995-3-31
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pubmed:abstractText |
We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0938-8990
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
688-95
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7873878-Animals,
pubmed-meshheading:7873878-Base Sequence,
pubmed-meshheading:7873878-Feasibility Studies,
pubmed-meshheading:7873878-Genes, Intracisternal A-Particle,
pubmed-meshheading:7873878-Genetic Markers,
pubmed-meshheading:7873878-Leukemia Virus, Murine,
pubmed-meshheading:7873878-Mice,
pubmed-meshheading:7873878-Mice, Inbred BALB C,
pubmed-meshheading:7873878-Mice, Inbred C3H,
pubmed-meshheading:7873878-Mice, Inbred C57BL,
pubmed-meshheading:7873878-Molecular Sequence Data,
pubmed-meshheading:7873878-Polymerase Chain Reaction,
pubmed-meshheading:7873878-Proviruses,
pubmed-meshheading:7873878-Repetitive Sequences, Nucleic Acid
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pubmed:year |
1994
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pubmed:articleTitle |
Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR.
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pubmed:affiliation |
National Institute for Medical Research, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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