Linked Life Data

7871749

Source:http://linkedlifedata.com/resource/pubmed/id/7871749

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-3-28
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81425, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81426, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81427, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81428, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81429, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81430, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81431, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81432, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81433, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81434, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81435, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81436, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X81437
pubmed:abstractText
NSP3, an acidic nonstructural protein, encoded by gene 7 has been implicated as the key player in the assembly of the 11 viral plus-strand RNAs into the early replication intermediates during rotavirus morphogenesis. To date, the sequence of NSP3 from only three animal rotaviruses (SA11, SA114F, and bovine UK) has been determined and that from a human strain has not been reported. To determine the genetic diversity among gene 7 alleles from group A rotaviruses, the nucleotide sequence of the NSP3 gene from 13 strains belonging to nine different G serotypes, from both humans and animals, has been determined. Based on the amino acid sequence identity as well as phylogenetic analysis, NSP3 from group A rotaviruses falls into three evolutionarily related groups, i.e., the SA11 group, the Wa group, and the S2 group. The SA11/SA114F gene appears to have a distant ancestral origin from that of the others and codes for a polypeptide of 315 amino acids (aa) in length. NSP3 from all other group A rotaviruses is only 313 aa in length because of a 2-amino-acid deletion near the carboxy-terminus. While the SA114F gene has the longest 3' untranslated region (UTR) of 132 nucleotides, that from other strains suffered deletions of varying lengths at two positions downstream of the translational termination codon. In spite of the divergence of the nucleotide (nt) sequence in the protein coding region, a stretch of about 80 nt in the 3' UTR is highly conserved in the NSP3 gene from all the strains. This conserved sequence in the 3' UTR might play an important role in the regulation of expression of the NSP3 gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
207
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-33
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3.
pubmed:affiliation
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't