Linked Life Data

7858261

Source:http://linkedlifedata.com/resource/pubmed/id/7858261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1995-3-22
pubmed:abstractText
Karyotypic detection of chromosomal 16 abnormalities classically associated with AML M4Eo can be difficult. Characterization of the two genes involved in the inv(16)(p13q22), CBF beta and MYH11, has allowed the detection of fusion transcripts by reverse-transcriptase polymerase chain reaction (RT-PCR). We have analyzed CBF beta-MYH11 fusion transcripts by RT-PCR in myelomonocytic leukemias, with or without eosinophilia, to determine whether their presence correlates with morphology. Fifty-three cases (11 AML M4Eo; 1 AML M4 with atypical abnormal eosinophils (AML M4 "Eo"); 29 AML M4; 8 AML M5; 3 CMML; and 1 AML M2 with eosinophilia) were analyzed. All 11 typical AML M4Eo were CBF beta-MYH11 positive. The single case of AML M4 with distinctive eosinophil abnormalities was negative by karyotype, RT-PCR and fluorescent in situ hybridization (FISH). Three of 29 (10%) AML M4 without abnormal eosinophils were CBF beta-MYH11 positive, 1 of which did not show any apparent chromosome 16 abnormalities by classical metaphase analysis (2 not tested). Both cases tested also showed MYH11 genomic rearrangement. None of the other leukemias were RT-PCR positive. Follow-up of three patient showed residual positivity in apparent complete remission. These data show that CBF beta-MYH11 fusion transcripts occur not only in the vast majority of typical AML M4Eo, but also in approximately 10% of AML M4 without eosinophilic abnormalities, a much higher incidence than the sporadic reports of chromosome 16 abnormalities in AML M4 would suggest. Taken together with the detection of CBF beta-MYH11 transcripts in the absence of apparent chromosome 16 abnormalities by classical banding techniques, these data show that additional screening by either RT-PCR or FISH should be performed in all AML M4, regardless of morphologic features, to allow accurate evaluation of the prognostic importance of this fusion transcript.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
85
pubmed:geneSymbol
AML1, MYH11, PEBP2&Bgr;
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
1313-22
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7858261-Adolescent, pubmed-meshheading:7858261-Adult, pubmed-meshheading:7858261-Aged, pubmed-meshheading:7858261-Base Sequence, pubmed-meshheading:7858261-Child, pubmed-meshheading:7858261-Child, Preschool, pubmed-meshheading:7858261-Chromosome Aberrations, pubmed-meshheading:7858261-Chromosome Inversion, pubmed-meshheading:7858261-Chromosomes, Human, Pair 16, pubmed-meshheading:7858261-Core Binding Factor Alpha 1 Subunit, pubmed-meshheading:7858261-Core Binding Factors, pubmed-meshheading:7858261-DNA-Binding Proteins, pubmed-meshheading:7858261-Eosinophils, pubmed-meshheading:7858261-Female, pubmed-meshheading:7858261-Humans, pubmed-meshheading:7858261-Infant, pubmed-meshheading:7858261-Leukemia, Myelomonocytic, Acute, pubmed-meshheading:7858261-Male, pubmed-meshheading:7858261-Middle Aged, pubmed-meshheading:7858261-Molecular Sequence Data, pubmed-meshheading:7858261-Myosins, pubmed-meshheading:7858261-Neoplasm Proteins, pubmed-meshheading:7858261-Oncogene Proteins, Fusion, pubmed-meshheading:7858261-Polymerase Chain Reaction, pubmed-meshheading:7858261-RNA, Messenger, pubmed-meshheading:7858261-RNA, Neoplasm, pubmed-meshheading:7858261-Transcription Factor AP-2, pubmed-meshheading:7858261-Transcription Factors
pubmed:year
1995
pubmed:articleTitle
Detection of the chromosome 16 CBF beta-MYH11 fusion transcript in myelomonocytic leukemias.
pubmed:affiliation
Department of Hematology, CHU Necker-Enfants Malades, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't