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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1995-3-10
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pubmed:abstractText |
Intact cells of Saccharomyces cerevisiae were examined as an aqueous paste by 13C-nmr spectroscopy with direct polarization and magic-angle spinning. The spectra obtained were highly resolved, showing numerous resonances in the 60-105 ppm range that were assigned to carbons of a liquid-like domain of the cell wall glucan. Assignments were confirmed by running the spectrum of S. cerevisiae in which the cell wall glucans were labeled with [13C] by feeding the cell [13C]galactose. The spectra indicate that the glucan in the cell wall of intact S. cerevisiae assumes a helical conformation and suggest that strain 17A fed with galactose preferentially incorporates the resulting glucose into beta (1-->3)-linkages.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-3525
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1627-35
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7849225-Carbohydrate Sequence,
pubmed-meshheading:7849225-Carbon Isotopes,
pubmed-meshheading:7849225-Cell Wall,
pubmed-meshheading:7849225-Glucans,
pubmed-meshheading:7849225-Magnetic Resonance Spectroscopy,
pubmed-meshheading:7849225-Molecular Sequence Data,
pubmed-meshheading:7849225-Saccharomyces cerevisiae
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pubmed:year |
1994
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pubmed:articleTitle |
Direct observation of cell wall glucans in whole cells of Saccharomyces cerevisiae by magic-angle spinning 13C-NMR.
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pubmed:affiliation |
Department of Chemistry, College of Staten Island, City University of New York 10314.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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