Linked Life Data

7828914

Source:http://linkedlifedata.com/resource/pubmed/id/7828914

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-2-23
pubmed:databankReference
pubmed:abstractText
In order to characterize individual protein components of the mitochondrial (mt) ribosome for regulatory, functional and evolutionary studies, the yeast nuclear gene MRP-L4 (accession No. Z30582), coding for the mt ribosomal protein (MRP) YmL4, has been cloned using oligodeoxyribonucleotides (oligos) deduced from a partial amino acid (aa) sequence [Graack et al., FEBS Lett. 242 (1988) 4-8] as screening probes. MRP-L4 is located on chromosome XII and codes for a slightly basic protein of 319 aa. The first 14 aa have not been found in the mature protein, and putatively form a signal peptide that is cleaved off during or after mt import. YmL4 has an N terminus very rich in Pro residues, and at its C terminus contains four hydrophobic domains. YmL4 shows no significant sequence similarity to any other sequence from the databases. Gene disruption shows the MRP-L4 product to be indispensable for mt function in cells growing on non-fermentable carbon sources. In contrast to nearly all other MRPs investigated so far, gene disruption of MRP-L4 also affects growth of yeast cells on fermentable carbon sources, suggesting additional cytosolic and/or mt functions of YmL4 besides its involvement in mt protein biosynthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
152
pubmed:geneSymbol
MRP-L4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
107-12
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:7828914-Amino Acid Sequence, pubmed-meshheading:7828914-Base Sequence, pubmed-meshheading:7828914-Cell Compartmentation, pubmed-meshheading:7828914-Chromosomes, Fungal, pubmed-meshheading:7828914-Cloning, Molecular, pubmed-meshheading:7828914-Genes, Fungal, pubmed-meshheading:7828914-Genes, Lethal, pubmed-meshheading:7828914-Mitochondria, pubmed-meshheading:7828914-Mitochondrial Proteins, pubmed-meshheading:7828914-Molecular Sequence Data, pubmed-meshheading:7828914-Mutagenesis, Insertional, pubmed-meshheading:7828914-Protein Sorting Signals, pubmed-meshheading:7828914-Ribosomal Proteins, pubmed-meshheading:7828914-Saccharomyces cerevisiae, pubmed-meshheading:7828914-Saccharomyces cerevisiae Proteins, pubmed-meshheading:7828914-Sequence Analysis, DNA, pubmed-meshheading:7828914-Sequence Homology, Amino Acid
pubmed:year
1995
pubmed:articleTitle
Gene MRP-L4, encoding mitochondrial ribosomal protein YmL4, is indispensable for proper non-respiratory cell functions in yeast.
pubmed:affiliation
Institut für Genetik, Freie Universität Berlin, Germany.
pubmed:publicationType
Journal Article, Comparative Study