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pubmed-article:7827503pubmed:abstractTextBoth subunits of the human class II transcription factor TFIIE (rTFIIE alpha and rTFIIE beta) have been overexpressed in Escherichia coli at 26 degrees C using a T7 RNA polymerase expression system and further purified to apparent homogeneity. As in this system rTFIIE alpha was poorly expressed and copurified with a truncated form, we expressed rTFIIE alpha as a fusion protein. These overexpressed subunits of TFIIE are similar to the endogenous subunits according to the following criteria: molecular weight, microsequencing, and transcription activity.lld:pubmed
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pubmed-article:7827503pubmed:articleTitleExpression in Escherichia coli: production and purification of both subunits of the human general transcription factor TFIIE.lld:pubmed
pubmed-article:7827503pubmed:affiliationLaboratoire de Génétique Moléculaire des Eucaryotes du CNRS, U184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.lld:pubmed
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