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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1995-2-21
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pubmed:abstractText |
Both subunits of the human class II transcription factor TFIIE (rTFIIE alpha and rTFIIE beta) have been overexpressed in Escherichia coli at 26 degrees C using a T7 RNA polymerase expression system and further purified to apparent homogeneity. As in this system rTFIIE alpha was poorly expressed and copurified with a truncated form, we expressed rTFIIE alpha as a fusion protein. These overexpressed subunits of TFIIE are similar to the endogenous subunits according to the following criteria: molecular weight, microsequencing, and transcription activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Affinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Histidine,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, TFII,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/bacteriophage T7 RNA polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/polyhistidine,
http://linkedlifedata.com/resource/pubmed/chemical/transcription factor TFIIE
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1046-5928
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
458-67
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7827503-Affinity Labels,
pubmed-meshheading:7827503-Amino Acid Sequence,
pubmed-meshheading:7827503-Base Sequence,
pubmed-meshheading:7827503-DNA-Directed RNA Polymerases,
pubmed-meshheading:7827503-Escherichia coli,
pubmed-meshheading:7827503-Histidine,
pubmed-meshheading:7827503-Humans,
pubmed-meshheading:7827503-Molecular Sequence Data,
pubmed-meshheading:7827503-Peptides,
pubmed-meshheading:7827503-Protein Conformation,
pubmed-meshheading:7827503-Recombinant Fusion Proteins,
pubmed-meshheading:7827503-Transcription Factors,
pubmed-meshheading:7827503-Transcription Factors, TFII,
pubmed-meshheading:7827503-Viral Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
Expression in Escherichia coli: production and purification of both subunits of the human general transcription factor TFIIE.
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pubmed:affiliation |
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, U184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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