Linked Life Data

7819150

Source:http://linkedlifedata.com/resource/pubmed/id/7819150

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1995-2-16
pubmed:abstractText
O-acetyl GD3 ganglioside is a cell surface molecule of some neural, neural crest and renal cells. Here we show, by using mAbs specific for O-acetyl GD3 (clone 27A) and flow-cytometric, biochemical or immunological techniques, that it is also expressed at high intensity level on the surface of 49.6% (median) of the CD3+ cells (T lymphocytes), at medium level in 16.2% of the CD16+ (natural killer) cells, at very low level in 51.9% of CD14+ cells (monocytes) and in 6.9% of CD20+ cells (B lymphocytes), but not in other human blood cells. Of the CD4+ or CD8+ cells, 52.6 or 36.5% respectively were 27A+. Furthermore, 81.6% of the CD45RO+ lymphocytes carried the O-acetyl GD3 ganglioside. It was not detected in the thymus, although its immediate precursor, the GD3 ganglioside, was present in the medullary thymocytes, suggesting that O-acetyltransferases are regulated by maturation events taking place in the periphery. The anti-O-acetyl GD3 antibodies induced a strong mitogenic response in cultured peripheral blood mononuclear cells, but not in purified T cells. However, in combination with phorbol myristate acetate the antibodies induced proliferation also in purified T cells, suggesting that protein kinase C priming is needed for this effect. This and the restricted expression of O-acetyl GD3 suggest a functional role for this ganglioside in T cell subpopulations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1409-16
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
O-acetyl GD3 ganglioside in human peripheral blood T lymphocytes.
pubmed:affiliation
Department of Bacteriology and Immunology, University of Helsinki, Finland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't