Linked Life Data

7812974

Source:http://linkedlifedata.com/resource/pubmed/id/7812974

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-2-3
pubmed:abstractText
We compared the properties of six human meningiomas with normal rat meningeal cells using cell culture techniques, high resolution in vitro 1H-NMR (nuclear magnetic resonance) spectroscopy, and chromatographic analysis. Cell cultures were immunocytochemically characterized at all stages with specific antibodies. Quantitative and qualitative metabolite assessments in cell extracts were obtained from 1H-NMR spectra and chromatographic analysis. Human meningioma cells expressed a characteristic spectrum of metabolites including free amino acids, compounds related to membrane phospholipid metabolism, energy metabolites, and other intermediary products. These spectral characteristics, although different in some respects, were strikingly similar to the ones of rat meningeal cells. Particularly, several metabolites that allow discrimination between meningeal cells and other cell types of the central nervous system were preserved in meningiomas. These similarities suggest that the regulation of intracellular levels of such metabolites is so intrinsic to the identity of cell type as to be conserved across species and through transformation. Additionally, human meningioma cultures expressed some spectroscopic characteristics that enabled them to be clearly distinguished from primary rat meningeal cultures. Thus, human meningiomas may be both specifically recognizable by 1H-NMR spectroscopy and also distinguishable from normal rat meningeal tissue. Our results raise the eventual possibility of using NMR in the noninvasive diagnosis of brain tumors in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
420-7
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Cell type-specific fingerprinting of meningioma and meningeal cells by proton nuclear magnetic resonance spectroscopy.
pubmed:affiliation
Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, United Kingdom.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't