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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1995-2-8
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pubmed:abstractText |
Phosphodiesterase (PDE) activities that were capable of hydrolysing cyclic AMP (Km = 6.8 +/- 2 microM) and cyclic GMP (Km = 6.7 +/- 1.6 microM) were isolated from tracheal smooth muscle. These enzyme(s) activities were insensitive to stimulation by calcium/calmodulin and to inhibition by cyclic GMP, rolipram (type IV inhibitor) and siguazodan (type III inhibitor). Zaprinast was a relatively poor inhibitor of both cyclic AMP and cyclic GMP hydrolysis (IC50 = 46 +/- 9 microM and 45 +/- 14 microM respectively). These results suggest that tracheal smooth muscle may contain an apparently novel PDE. However, KCl (30 mM) which facilitates calcium entry in cells, depressed bradykinin-stimulated intracellular cyclic AMP formation, suggesting that the type I PDE may be functionally present. We suggest that considerable caution be exercised in identifying apparently novel PDE isoforms.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7812625-3',5'-Cyclic-AMP Phosphodiesterases,
pubmed-meshheading:7812625-3',5'-Cyclic-GMP Phosphodiesterases,
pubmed-meshheading:7812625-Animals,
pubmed-meshheading:7812625-Bradykinin,
pubmed-meshheading:7812625-Guinea Pigs,
pubmed-meshheading:7812625-Isoenzymes,
pubmed-meshheading:7812625-Muscle, Smooth,
pubmed-meshheading:7812625-Potassium Chloride,
pubmed-meshheading:7812625-Trachea
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pubmed:year |
1994
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pubmed:articleTitle |
The identification of apparently novel cyclic AMP and cyclic GMP phosphodiesterase activities in guinea-pig tracheal smooth muscle.
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pubmed:affiliation |
Department of Physiology and Pharmacology, Strathclyde University, Glasgow.
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pubmed:publicationType |
Journal Article,
In Vitro
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