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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6 Pt 2
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pubmed:dateCreated |
1995-1-27
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pubmed:abstractText |
24-Hydroxylase has been considered a major enzyme regulating metabolism of circulating 1 alpha, 25-dihydroxyvitamin D3 [1,25(OH)2D3]. To understand the metabolism of 1,25(OH)2D3 in chronic renal failure, we examined 1,25(OH)2D3-induced 25-hydroxyvitamin D3-24-hydroxylase (24-hydroxylase) gene expression in the intestine of uremic rats. Northern blot and dot blot analyses showed that the induction of duodenal 24-hydroxylase gene expression was 2.0- to 3.8-fold greater in uremic rats than in sham-operated rats (P < 0.05, Student's t-test) at 6 h after 1,25(OH)2D3 administration. Gene induction of calbindin D9k by 1,25(OH)2D3 was not augmented in uremic group. In situ hybridization analysis revealed that the induction of 24-hydroxylase mRNA by 1,25(OH)2D3 was observed exclusively in the columnar epithelium of the crypt and the lower part of the villi, suggesting that the stage of epithelial cell differentiation is a major determinant of 1,25(OH)2D3-induced 24-hydroxylase gene expression. In uremia, 1,25(OH)2D3-induced 24-hydroxylase gene expression was accelerated selectively, possibly because of poorly differentiated epithelial cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Steroid Hydroxylases,
http://linkedlifedata.com/resource/pubmed/chemical/vitamin D 24-hydroxylase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-9513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F926-30
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pubmed:dateRevised |
2008-8-16
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pubmed:meshHeading |
pubmed-meshheading:7810699-Animals,
pubmed-meshheading:7810699-Blotting, Northern,
pubmed-meshheading:7810699-Calcitriol,
pubmed-meshheading:7810699-Cytochrome P-450 Enzyme System,
pubmed-meshheading:7810699-Duodenum,
pubmed-meshheading:7810699-Gene Expression,
pubmed-meshheading:7810699-In Situ Hybridization,
pubmed-meshheading:7810699-Intestines,
pubmed-meshheading:7810699-RNA, Messenger,
pubmed-meshheading:7810699-Rats,
pubmed-meshheading:7810699-Steroid Hydroxylases,
pubmed-meshheading:7810699-Uremia
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pubmed:year |
1994
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pubmed:articleTitle |
Potentiated 1,25(OH)2D3-induced 24-hydroxylase gene expression in uremic rat intestine.
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pubmed:affiliation |
Second Department of Internal Medicine, Osaka City University Medical School, Japan.
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pubmed:publicationType |
Journal Article
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