Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-1-24
|
| pubmed:abstractText |
Neurons from the granular layer of the cerebellum express functional beta 2-adrenoreceptors (beta 2-ARs). We show that stimulation of beta 2-ARs with isoprenaline increases cyclic AMP (cAMP) production and stimulates transcription of genes containing the cAMP-responsive element (CRE; TGACGTCA). This effect is mediated by cAMP-dependent protein kinase and the trans-acting factor CRE binding protein. Transcriptional regulation by the beta 2-AR was investigated by using the c-fos protooncogene as a model system. We show that beta 2-ARs stimulate c-fos mRNA accumulation, increase AP1 binding activity, and stimulate transcription through the phorbol ester-responsive element (TGACTCA). The transcriptional regulation of c-fos itself was studied with reporter constructs driven by c-fos promoter sequences. Deletion studies revealed that beta 2-ARs stimulate c-fos transcription through at least three distinct regulatory sequences: (a) the CRE located at -60 bp 5' to the initiation site, (b) the fos AP1-like element (-291 to -297), and (c) the serum-responsive element (-297 to -317). The regulation of these elements by the two putative second messengers of the beta 2-AR, cAMP and Ca2+, was analyzed. We report that all three of these regulatory sequences are coregulated by both second messengers. These results indicate that beta 2-ARs stimulate c-fos transcription by multiple cAMP- and Ca(2+)-dependent regulatory elements in neurons.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, beta-2,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
64
|
| pubmed:geneSymbol |
c-fos
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
41-51
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7798940-Animals,
pubmed-meshheading:7798940-Base Sequence,
pubmed-meshheading:7798940-Calcium,
pubmed-meshheading:7798940-Cells, Cultured,
pubmed-meshheading:7798940-Cerebellum,
pubmed-meshheading:7798940-Cyclic AMP,
pubmed-meshheading:7798940-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:7798940-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:7798940-Gene Expression,
pubmed-meshheading:7798940-Genes, fos,
pubmed-meshheading:7798940-Isoproterenol,
pubmed-meshheading:7798940-Molecular Sequence Data,
pubmed-meshheading:7798940-Neurons,
pubmed-meshheading:7798940-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:7798940-RNA, Messenger,
pubmed-meshheading:7798940-Rats,
pubmed-meshheading:7798940-Receptors, Adrenergic, beta-2,
pubmed-meshheading:7798940-Second Messenger Systems,
pubmed-meshheading:7798940-Transcription, Genetic,
pubmed-meshheading:7798940-Transcription Factors
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Beta 2-adrenoreceptors stimulate c-fos transcription through multiple cyclic AMP- and Ca(2+)-responsive elements in cerebellar granular neurons.
|
| pubmed:affiliation |
Institut de Physiologie et de Chimie Biologique, URA 1446 du CNRS, Strasbourg, France.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|