Linked Life Data

7789994

Source:http://linkedlifedata.com/resource/pubmed/id/7789994

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-7-21
pubmed:abstractText
Expanded blocks of repeating nucleotide triplets have been found in or near genes associated with several human diseases. In the case of myotonic dystrophy, a block of repeating CTG trinucleotides is located downstream of the gene, and expansions of this block to repeats of n = 100 or more are frequently found in afflicted individuals. Using electron microscopy, we recently demonstrated that these blocks form unusually stable nucleosomes. Here, competitive nucleosome reconstitution was employed to measure the energetics of nucleosome formation over CTG repeat blocks of n = 75 and n = 130. These values were compared to the Xenopus borealis somatic 5S RNA gene, previously one of the strongest known natural nucleosome positioning elements. It is shown that DNA fragments containing 75 and 130 CTG repeats are 6 and 9 times stronger in nucleosome formation, respectively, than the 5S RNA gene. These findings suggest that expanded CTG blocks may profoundly alter local chromatin structure.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
570-3
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Expanded CTG triplet blocks from the myotonic dystrophy gene create the strongest known natural nucleosome positioning elements.
pubmed:affiliation
Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599-7295, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't