Linked Life Data

7780823

Source:http://linkedlifedata.com/resource/pubmed/id/7780823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-7-14
pubmed:abstractText
For developing monoclonal antibodies (MAb) that bound to lipid A of the LPS molecule, we established several hybridomas cell lines by fusion of SP2/0 and spleen cells from BALB/c mice immunized by J5 mutant strain of E. coli 0111:B4. Each MAb exhibited a high titer by ELISA assay. Six of MAb were of immunoglobulin G isotype and another one immunoglobulin M. All of these MAb demonstrated different capacity to cross-react with several gram-negative bacteria and their endotoxins. Among them, 9G6 MAb improved the survival rate of mice significantly when administered 2 hours before the challenge by pseudomonas A and E. coli. These findings suggest that MAb will probably be useful in the treatment of sepsis caused by a wide variety of gram-negative bacteria.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0376-2491
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-60, 190-1
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
[Establishment of hybridoma lines and protective study of monoclonal antibodies against lipid A of endotoxins].
pubmed:affiliation
Institute of Burn Research, Southwestern Hospital, Third Military Medical College, Chongqing.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't