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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1995-7-12
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pubmed:abstractText |
Granzyme (Gzm) B-deficient mice obtained by gene targeting were used to assess the role of Gzm B in the mechanisms used by natural killer (NK) and lymphokine-activated killer (LAK) cells to destroy target cells. Gzm B-/- NK cells, LAK cells, and cytotoxic T lymphocytes (CTL) all are defective in their ability to rapidly induce DNA fragmentation/apoptosis in susceptible target cells. This defect can be partially corrected with long incubation times of effector and target cells. Moreover, Gzm B-/- NK cells (but not CTL or LAK cells) exhibit a defect in 51Cr release from susceptible target cells. This 51Cr release defect in Gzm B-deficient NK cells is also not overcome by prolonged incubation times or high effector-to-target cell ratios. We conclude that Gzm B plays a critical and nonredundant role in the rapid induction of DNA fragmentation/apoptosis by NK cells, LAK cells, and CTL. Gzm B may have an additional role in NK cells (but not in CTL or LAK cells) for mediating 51Cr release.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-1423596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-1460287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-1460416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-1603092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-1732416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-2417226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-2788710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-2794864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-313007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-3134294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-3292396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-3873011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-3874868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-3904772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-6347870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-6445041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-6977583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7085883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7507956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7518614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7520535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7526382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7533644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7678113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7882166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-7972104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-8108732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-8133042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-8137431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-8164737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-8245461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7777569-8452680
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5679-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7777569-Amino Acid Sequence,
pubmed-meshheading:7777569-Animals,
pubmed-meshheading:7777569-Apoptosis,
pubmed-meshheading:7777569-Cells, Cultured,
pubmed-meshheading:7777569-Cytotoxicity, Immunologic,
pubmed-meshheading:7777569-Granzymes,
pubmed-meshheading:7777569-Killer Cells, Lymphokine-Activated,
pubmed-meshheading:7777569-Killer Cells, Natural,
pubmed-meshheading:7777569-Mice,
pubmed-meshheading:7777569-Molecular Sequence Data,
pubmed-meshheading:7777569-Serine Endopeptidases
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pubmed:year |
1995
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pubmed:articleTitle |
Natural killer and lymphokine-activated killer cells require granzyme B for the rapid induction of apoptosis in susceptible target cells.
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pubmed:affiliation |
Department of Medicine, Washington University Medical School, St. Louis, MO 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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