Linked Life Data

7775326

Source:http://linkedlifedata.com/resource/pubmed/id/7775326

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1995-7-11
pubmed:abstractText
The hypothesis that hypoxic excitation is coupled to dopamine (DA) secretion was tested in perfused-superfused cat carotid bodies (CB). DA was electrochemically detected by an amperometric method (constant applied potential +150 mV) with Nafion polymer-coated recessed gold microsensors (tip diameter 3-8 microns) in 10 cat CBs. Neural discharge (ND) from the whole sinus nerve was measured simultaneously with DA changes during interruption of perfusate flow and during hypoxic perfusion (5% O2). A computer-controlled instrument using a chronoamperometric technique (+550-mV pulses) with a Nafion-coated carbon fiber microelectrode (tip diameter 35 microns) was used to detect DA changes in two CBs during similar hypoxic stimuli. Rapid DA release kinetics were measured during flow interruption with an initial rate of 1.05 +/- 0.15 (SE) microM/s within the first 10-15 s. At most measurement sites, the increase in DA preceded the rise in ND. After the initial increase, DA release slowed to 0.16 +/- 0.02 microM/s, reaching a maximum DA concentration of 20.7 +/- 2.6 microM above baseline after 90 s of flow interruption. Nicotine (10-micrograms bolus) caused a large increase in ND without a proportional increase in DA release. Spatially detailed time-resolved electrochemical measurements were able to discriminate between DA release during hypoxia and chemoexcitatory responses that do not involve DA release.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
8750-7587
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
830-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Electrochemical detection of rapid DA release kinetics during hypoxia in perfused-superfused cat CB.
pubmed:affiliation
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.