Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1995-7-5
pubmed:abstractText
The FliM protein of Escherichia coli is essential for the assembly and function of flagella. Here, we report the effects of controlled low-level expression of FliM in a fliM null strain. Disruption of the fliM gene abolishes flagellation. Underexpression of FliM causes cells to produce comparatively few flagella, and most flagella built are defective, producing subnormal average torque and fluctuating rapidly in speed. The results imply that in a normal flagellar motor, multiple molecules of FliM are present and can function independently to some degree. The speed fluctuations indicate that stable operation requires most, possibly all, of the normal complement of FliM. Thus, the FliM subunits are not as fully independent as the motility proteins MotA and MotB characterized in earlier work, suggesting that FliM occupies a location in the motor distinct from the MotA/MotB torque generators. Several mutations in fliM previously reported to cause flagellar paralysis in Salmonella typhimurium (H. Sockett, S. Yamaguchi, M. Kihara, V.M. Irikura, and R. M. Macnab, J. Bacteriol. 174:793-806, 1992) were made and characterized in E. coli. These mutations did not cause flagellar paralysis in E. coli; their phenotypes were more complex and suggest that FliM is not directly involved in torque generation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1458024, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1631080, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1631122, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1640458, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1732214, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1882727, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-1938906, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2061285, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2154333, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2407857, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2447650, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2537812, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2610349, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2849208, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-2984180, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-3007433, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-3023634, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-3050128, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-3536867, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-3537305, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-3843705, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-4593496, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-4927675, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-6374467, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-6396378, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-7007337, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-7892209, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-7919791, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-8107139, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-8224881, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-8308888, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-8415608, http://linkedlifedata.com/resource/pubmed/commentcorrection/7768858-8423152
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3485-95
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Regulated underexpression of the FliM protein of Escherichia coli and evidence for a location in the flagellar motor distinct from the MotA/MotB torque generators.
pubmed:affiliation
Department of Biology, University of Utah, Salt Lake City 84112, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.