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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1995-7-5
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pubmed:abstractText |
The sigma S subunit of RNA polymerase (encoded by the rpoS gene) is the master regulator in a complex regulatory network that controls stationary-phase induction and osmotic regulation of many genes in Escherichia coli. Here we demonstrate that the histone-like protein H-NS is also a component of this network, in which it functions as a global inhibitor of gene expression during the exponential phase of growth. On two-dimensional gels, at least 22 sigma S-controlled proteins show increased expression in an hns mutant. H-NS also inhibits the expression of sigma S itself by a mechanism that acts at the posttranscriptional level. Our results indicate that relief of repression by H-NS plays a role in stationary-phase induction as well as in hyperosmotic induction of rpoS translation. Whereas certain sigma S-dependent genes (e.g., osmY) are only indirectly regulated by H-NS via its role in the control of sigma S expression, others are also H-NS-regulated in a sigma S-independent manner. (For this latter class of genes, rpoS hns double mutants show higher levels of expression than mutants deficient in rpoS alone.) In addition, we demonstrate that the slow-growth phenotype of hns mutants is suppressed in hns rpoS double mutants and that many second-site suppressor mutants that spontaneously arise from hns strains carry lesions that affect the expression of sigma S.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1310094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1324388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1385595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1423593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1648559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1658595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1744047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1745240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1825082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-1849609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-2014002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-2128526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-2128918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-2254248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-2404280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-2830029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-3131312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-6379600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7525405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7816034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7854117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7859747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7934831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7984087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-7984106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8021175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8057848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8071219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8071220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8120010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8206842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8208244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8218173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8253679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8282684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8306968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8355613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8416901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8425216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8455549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8458322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8458856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8458857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8459772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7768855-8475100
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3455-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7768855-Bacterial Outer Membrane Proteins,
pubmed-meshheading:7768855-Bacterial Proteins,
pubmed-meshheading:7768855-Base Sequence,
pubmed-meshheading:7768855-Cell Division,
pubmed-meshheading:7768855-DNA-Binding Proteins,
pubmed-meshheading:7768855-Escherichia coli,
pubmed-meshheading:7768855-Gene Expression Regulation, Bacterial,
pubmed-meshheading:7768855-Molecular Sequence Data,
pubmed-meshheading:7768855-Mutation,
pubmed-meshheading:7768855-Osmolar Concentration,
pubmed-meshheading:7768855-Sigma Factor
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pubmed:year |
1995
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pubmed:articleTitle |
Role for the histone-like protein H-NS in growth phase-dependent and osmotic regulation of sigma S and many sigma S-dependent genes in Escherichia coli.
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pubmed:affiliation |
Department of Biology, University of Konstanz, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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