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rdf:type |
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lifeskim:mentions |
|
|
pubmed:issue |
9
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|
pubmed:dateCreated |
1993-10-14
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pubmed:abstractText |
We have engineered mammalian cell lines to produce high levels of heterologous proteins by constructing a cell line that expresses the herpesvirus transactivator, VP16. Subsequent stable transfection with a gene of interest under control of a herpesvirus immediate early promoter led to a rapid isolation of cell lines producing between 1 and 20 micrograms of protein/million cells/24 hours. This high level expression is stable for at least five months.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
B
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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|
pubmed:issn |
0733-222X
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
|
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1037-41
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7764072-Animals,
pubmed-meshheading:7764072-Base Sequence,
pubmed-meshheading:7764072-Blotting, Northern,
pubmed-meshheading:7764072-CHO Cells,
pubmed-meshheading:7764072-Cell Line,
pubmed-meshheading:7764072-Cricetinae,
pubmed-meshheading:7764072-Growth Hormone,
pubmed-meshheading:7764072-Herpes Simplex Virus Protein Vmw65,
pubmed-meshheading:7764072-Kidney,
pubmed-meshheading:7764072-Molecular Sequence Data,
pubmed-meshheading:7764072-Papillomaviridae,
pubmed-meshheading:7764072-Plasmids,
pubmed-meshheading:7764072-Protein Engineering,
pubmed-meshheading:7764072-Recombinant Proteins,
pubmed-meshheading:7764072-Tissue Plasminogen Activator,
pubmed-meshheading:7764072-Transfection
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pubmed:year |
1993
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pubmed:articleTitle |
High level, stable production of recombinant proteins in mammalian cell culture using the herpesvirus VP16 transactivator.
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pubmed:affiliation |
Monsanto Company, St. Louis, MO 63198.
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pubmed:publicationType |
Journal Article,
Comparative Study
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