Linked Life Data

7763702

Source:http://linkedlifedata.com/resource/pubmed/id/7763702

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-7-30
pubmed:abstractText
We examined the differential replication of Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV; Family: Baculoviridae) in Spodoptera frugiperda (SF) and Bombyx mori (BM) cell lines. Our previous studies have shown that infection of BM cells is abortive; infectious progeny is not produced, and although virogenic stroma is produced, complete virions or inclusion bodies are not assembled. We designed the present study to determine the extent of infected-cell-specific polypeptides (ICSPs) and viral DNA synthesis in the abortive infection and to see if restriction of virus replication in BM cells is due to a block at the transcriptional level. Pulse-labeling studies showed that a majority of the ICSPs detected in productive SF cells were not synthesized in BM cells. Many of these ICSPs were virion components. Viral DNA replicated in BM cells but more slowly and at a much lower level than in SF cells. Northern blot analysis showed that i) AcMNPV immediate-early gene (IE-1) was transcribed in BM cells at higher levels than in SF cells; ii) a delayed-early gene (dnapol) and a late gene (cap) were transcribed at much lower levels in the abortive infection; and iii) a very late gene (polh) was transcribed at extremely low levels in BM cells. We conclude that virus replication in BM cells is primarily restricted during or prior to the delayed-early stage and occurs at the transcriptional level. It appears that the late and very late effects observed are consequences of the primary block.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1052-6781
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Host-specific transcription of baculovirus genes.
pubmed:affiliation
Department of Biological Sciences, Texas Tech University, Lubbock 79409-3131.
pubmed:publicationType
Journal Article