Linked Life Data

77628

Source:http://linkedlifedata.com/resource/pubmed/id/77628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1978-7-26
pubmed:abstractText
Localization of actin-like protein in the mouse brain was studied by the immunofluorescence method using FITC-labeled antiactin antibody. Antibody against actin was prepared according to Lazarides (1974). The problem of poor antigenicity and the presence of contaminants in the antigen preparation was circumvented by way of denaturation of the protein with sodium dodecyl sulfate (SDS) and subsequent purification of the antigen by SDS polyacrylamide gel electrophoresis (Lazarides, 1974). Intense specific fluorescence against actin was noted in the cytoplasma of the epithelial cells of the choroid plexus, of the ependymal cells of the ventricles, and a little less one in arachnoidea and in leptomeninges, as well as in the muscle layers, endothelial linings, and adventitia of the arteries. Diffuse but weak fluorescence was noted also in the neuropil of the grey matter of the brain. Among the latter, fluorescence in the granular layer of Ammon's horn and of the cerebellum appeared clearer than the diffuse one in the rest of the grey matter. Functional significance of the presence of actin-like protein in the above tissues was discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0001-6322
pubmed:author
pubmed:issnType
Print
pubmed:day
24
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-103
pubmed:dateRevised
2007-11-9
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Immunofluorescence studies on localization of actin-like protein in the mouse brain.
pubmed:publicationType
Journal Article