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rdf:type |
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lifeskim:mentions |
umls-concept:C0003842,
umls-concept:C0017262,
umls-concept:C0018270,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0035696,
umls-concept:C0337112,
umls-concept:C1135918,
umls-concept:C1171362,
umls-concept:C1257890,
umls-concept:C1515670,
umls-concept:C1524075
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pubmed:issue |
6
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pubmed:dateCreated |
1995-6-27
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pubmed:databankReference |
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pubmed:abstractText |
Proliferation of smooth muscle cells (SMCs) and formation of a neointima are characteristics of the response of rat carotid arteries to balloon injury. Rat platelet-derived growth factor (PDGF)-B was cloned, thus allowing us to use species-specific probes to carry out in situ hybridization on the surface of injured arteries. A distinct population of luminal SMCs (7% to 10%) in the developing neointima expressed PDGF-B mRNA, but very few luminal SMCs still expressed PDGF-B (0.5%) when the lesion had stopped growing. Primary SMC cultures revealed expression of PDGF-B mRNA in 1.6% of SMCs derived from normal tunica media and in 11% of SMCs derived from the neointima. These data demonstrate that SMCs in the injured vessel wall are heterogeneous with regard to PDGF-B expression and that subculturing of these cells may give rise to cultures that are either positive or negative for PDGF-B expression. Furthermore, with abundant expression of the PDGF receptor beta-subunit expressed by intimal SMCs, our findings provide evidence that PDGF-B synthesized by these cells may be involved in intimal lesion formation via a paracrine or autocrine mechanism.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0009-7330
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
951-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7758166-Amino Acid Sequence,
pubmed-meshheading:7758166-Angioplasty, Balloon,
pubmed-meshheading:7758166-Animals,
pubmed-meshheading:7758166-Base Sequence,
pubmed-meshheading:7758166-Carotid Arteries,
pubmed-meshheading:7758166-Carotid Artery Injuries,
pubmed-meshheading:7758166-Cells, Cultured,
pubmed-meshheading:7758166-Cloning, Molecular,
pubmed-meshheading:7758166-DNA Probes,
pubmed-meshheading:7758166-Gene Expression,
pubmed-meshheading:7758166-In Situ Hybridization,
pubmed-meshheading:7758166-Male,
pubmed-meshheading:7758166-Molecular Sequence Data,
pubmed-meshheading:7758166-Muscle, Smooth, Vascular,
pubmed-meshheading:7758166-Platelet-Derived Growth Factor,
pubmed-meshheading:7758166-Population,
pubmed-meshheading:7758166-Protein-Tyrosine Kinases,
pubmed-meshheading:7758166-Proto-Oncogene Proteins,
pubmed-meshheading:7758166-Proto-Oncogene Proteins c-sis,
pubmed-meshheading:7758166-RNA, Messenger,
pubmed-meshheading:7758166-Rats,
pubmed-meshheading:7758166-Rats, Sprague-Dawley,
pubmed-meshheading:7758166-Receptors, Platelet-Derived Growth Factor
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pubmed:year |
1995
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pubmed:articleTitle |
A subpopulation of smooth muscle cells in injured rat arteries expresses platelet-derived growth factor-B chain mRNA.
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pubmed:affiliation |
University of Washington, Department of Pathology, Seattle 98195, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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