Linked Life Data

7756867

Source:http://linkedlifedata.com/resource/pubmed/id/7756867

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-6-26
pubmed:abstractText
A liquid chromatographic (LC) method employing prechromatographic oxidation for the determination of paralytic shellfish poison (PSP) toxins was evaluated. A number of changes to an earlier method resulted in improved separation and quantitation of most PSP analogues. Modification of the periodate oxidation reaction for the N-hydroxy-containing toxins led to improved sensitivity and stability of the products, enabling automated overnight analyses. These changes also enabled quantitation of gonyautoxins 1 and 4 (together) in the presence of gonyautoxins 2 and 3. Decarbamoylsaxitoxin can be identified and quantitated after peroxide oxidation. A cleanup step using a strong-anion-exchange column removed the C toxins and B-2 from the extracts and enabled a more accurate quantitation of gonyautoxins 1 and 4 and neosaxitoxin. Chiral chromatography, employing a reversed-phase column and chiral mobile-phase additives (copper-proline complex), was briefly evaluated for the separation of the oxidation products of the isomer pairs, gonyautoxins 1 and 4 and gonyautoxins 2 and 3. A comparison of the method with the mouse bioassay for the determination of PSP in lobster hepatopancreas (58 samples) showed a reasonable correlation (0.90) over a concentration range of 40-500 micrograms/100 g (saxitoxin equivalents), although the LC results were consistently higher than the mouse bioassay values by about 40%.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1060-3271
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
514-20
pubmed:dateRevised
2008-3-17
pubmed:meshHeading
pubmed:articleTitle
Evaluation of prechromatographic oxidation for liquid chromatographic determination of paralytic shellfish poisons in shellfish.
pubmed:affiliation
Health Protection Branch, Food Research Division, Ottawa, ON, Canada.
pubmed:publicationType
Journal Article, Comparative Study