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pubmed-article:7738146pubmed:abstractTextA manual non-radioactive DNA sequencing protocol was developed for rapid analysis of variable HIV-1 genomes. Sets of up to ten primers were used in one sequencing reaction. After polyacrylamide gel electrophoresis and blotting onto nylon membranes the individual sequences were detected by hybridization with digoxigenin-labelled oligonucleotides and chemiluminescence. The method is applicable to any sequencing project where numerous variants of DNA fragments of several 1000 bp of length are to be analysed.lld:pubmed
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pubmed-article:7738146pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:7738146pubmed:articleTitleA non-radioactive multiprime sequencing method for HIV genomes.lld:pubmed
pubmed-article:7738146pubmed:affiliationInstitute for Medical Microbiology, University of Regensburg, Germany.lld:pubmed
pubmed-article:7738146pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7738146pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed