Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
|
| pubmed:dateCreated |
1995-6-6
|
| pubmed:abstractText |
A manual non-radioactive DNA sequencing protocol was developed for rapid analysis of variable HIV-1 genomes. Sets of up to ten primers were used in one sequencing reaction. After polyacrylamide gel electrophoresis and blotting onto nylon membranes the individual sequences were detected by hybridization with digoxigenin-labelled oligonucleotides and chemiluminescence. The method is applicable to any sequencing project where numerous variants of DNA fragments of several 1000 bp of length are to be analysed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0166-0934
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
51
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
259-66
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7738146-DNA Primers,
pubmed-meshheading:7738146-DNA Probes,
pubmed-meshheading:7738146-Genetic Variation,
pubmed-meshheading:7738146-Genome, Viral,
pubmed-meshheading:7738146-HIV-1,
pubmed-meshheading:7738146-Humans,
pubmed-meshheading:7738146-Luminescent Measurements,
pubmed-meshheading:7738146-Molecular Probe Techniques,
pubmed-meshheading:7738146-Sequence Analysis, DNA
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
A non-radioactive multiprime sequencing method for HIV genomes.
|
| pubmed:affiliation |
Institute for Medical Microbiology, University of Regensburg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|