Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1995-6-2
pubmed:databankReference
pubmed:abstractText
The genes encoding toxic phospholipases D (PLD) from Corynebacterium pseudotuberculosis (Cp)biovar equi and C. ulcerans (Cu) have been cloned and sequenced. The deduced proteins are 307 amino acids (aa) in length and include a putative signal sequences of 26-aa. A molecular mass of 31.2 and 31.0 kDa and pI values of 8.84 and 6.73 are predicted for the secreted (mature) proteins from Cp and Cu, respectively. Comparison of the deduced primary structure of the two proteins to those of the PLD produced by Cp biovar ovis and Arcanobacterium haemolyticum (Ah) revealed that the four enzymes share 64-97% identity. The aa sequences of this group of proteins were unique when compared to the sequences of other phospholipases in GenBank and were found to share only small regions of homology with other proteins, including two conserved domains of glyceraldehyde-3-phosphate dehydrogenase (G3PD). The similarity of PLD from Cp biovar equi, Cu and Ah to the PLD of Cp biovar ovis suggests that these enzymes may act as virulence determinants.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
156
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
113-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7737503-Actinomycetaceae, pubmed-meshheading:7737503-Amino Acid Sequence, pubmed-meshheading:7737503-Base Sequence, pubmed-meshheading:7737503-Cloning, Molecular, pubmed-meshheading:7737503-Corynebacterium, pubmed-meshheading:7737503-Corynebacterium pseudotuberculosis, pubmed-meshheading:7737503-DNA, Bacterial, pubmed-meshheading:7737503-Escherichia coli, pubmed-meshheading:7737503-Gene Expression, pubmed-meshheading:7737503-Genes, Bacterial, pubmed-meshheading:7737503-Molecular Sequence Data, pubmed-meshheading:7737503-Molecular Weight, pubmed-meshheading:7737503-Phospholipase D, pubmed-meshheading:7737503-Restriction Mapping, pubmed-meshheading:7737503-Sequence Alignment, pubmed-meshheading:7737503-Sequence Analysis, DNA, pubmed-meshheading:7737503-Sequence Homology, Amino Acid
pubmed:year
1995
pubmed:articleTitle
Toxic phospholipases D of Corynebacterium pseudotuberculosis, C. ulcerans and Arcanobacterium haemolyticum: cloning and sequence homology.
pubmed:affiliation
Department of Veterinary Science, University of Arizona, Tucson 85721, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.