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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1995-6-8
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pubmed:abstractText |
We recently identified a differentiation inhibiting factor (I-factor) in mouse myeloid leukemia M1 cells as a murine homolog of nm23-H2/nucleoside diphosphate kinase (NDPK)-B gene product. We examined the I-factor activities of several authentic nm23/NDPK proteins, i.e. recombinant rat NDPK alpha and beta, recombinant mouse nm23-M1 and -M2, and recombinant human nm23-H1 and -H2 containing a mutant nm23-H2His protein lacing NDPK activity. Almost all these nm23/NDPK proteins showed I-factor activity. Moreover, to understand the active domain exhibiting I-factor activity of nm23-H2 protein lacking NDPK activity, we have investigated the I-factor activities of some truncated nm23-H2 proteins. The truncated nm23-H2 protein containing N-terminal peptide 1-60 retained the I-factor activity. These results provide the first evidence for a function of nm23/NDPK as a differentiation inhibiting factor in leukemic cells, that is independent of its NDPK activity and dependent on the presence of N-terminal peptide.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Monomeric GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NM23 Nucleoside Diphosphate Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NME1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nme1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoside-Diphosphate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
363
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
311-5
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7737424-Animals,
pubmed-meshheading:7737424-Base Sequence,
pubmed-meshheading:7737424-Biological Markers,
pubmed-meshheading:7737424-Cell Differentiation,
pubmed-meshheading:7737424-DNA Primers,
pubmed-meshheading:7737424-Humans,
pubmed-meshheading:7737424-Leukemia, Myeloid,
pubmed-meshheading:7737424-Mice,
pubmed-meshheading:7737424-Molecular Sequence Data,
pubmed-meshheading:7737424-Monomeric GTP-Binding Proteins,
pubmed-meshheading:7737424-NM23 Nucleoside Diphosphate Kinases,
pubmed-meshheading:7737424-Nucleoside-Diphosphate Kinase,
pubmed-meshheading:7737424-Rats,
pubmed-meshheading:7737424-Recombinant Proteins,
pubmed-meshheading:7737424-Sequence Deletion,
pubmed-meshheading:7737424-Structure-Activity Relationship,
pubmed-meshheading:7737424-Transcription Factors,
pubmed-meshheading:7737424-Tumor Cells, Cultured
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pubmed:year |
1995
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pubmed:articleTitle |
A new function of Nm23/NDP kinase as a differentiation inhibitory factor, which does not require it's kinase activity.
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pubmed:affiliation |
Department of Chemotherapy, Saitama Cancer Center Research Institute, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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