Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1995-5-31
|
| pubmed:abstractText |
Interleukin-3 (IL-3) stimulates colony formation of multiple lineages of hematopoietic cells. Bone marrow cells of A/J mice are nonresponsive to IL-3, and this observation has recently been correlated with aberrant mRNA splicing and impaired expression of the IL-3 receptor alpha subunit (IL-3R alpha), a binding component of the high-affinity receptors. We examined the IL-3R alpha gene in 27 inbred mouse strains and found the identical mutation, a 5-bp deletion at the branch point of intron 7, in 10 of these mouse strains. Bone marrow cells isolated from these 10 mouse strains did not express IL-3R alpha on the cell surface and did not form colonies in response to IL-3. Because the defective IL-3R alpha gene was found in several distantly related mouse strains, it appears to be a recessive allele rather than a sporadic mutation. In contrast, only 1 of 21 wild-derived mouse strains carried the 5-bp deletion in the IL-3R alpha gene. This study suggests that IL-3 function is not required for normal hematopoiesis in mice, but the retention of the IL-3 and IL-3R system may be of some selective advantage in wild populations.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
85
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2331-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7727767-Alleles,
pubmed-meshheading:7727767-Animals,
pubmed-meshheading:7727767-Base Sequence,
pubmed-meshheading:7727767-Bone Marrow,
pubmed-meshheading:7727767-Colony-Forming Units Assay,
pubmed-meshheading:7727767-DNA Mutational Analysis,
pubmed-meshheading:7727767-Drug Resistance,
pubmed-meshheading:7727767-Female,
pubmed-meshheading:7727767-Genes,
pubmed-meshheading:7727767-Genes, Recessive,
pubmed-meshheading:7727767-Hematopoietic Stem Cells,
pubmed-meshheading:7727767-Interleukin-3,
pubmed-meshheading:7727767-Mice,
pubmed-meshheading:7727767-Mice, Inbred A,
pubmed-meshheading:7727767-Mice, Inbred Strains,
pubmed-meshheading:7727767-Molecular Sequence Data,
pubmed-meshheading:7727767-Phenotype,
pubmed-meshheading:7727767-Phylogeny,
pubmed-meshheading:7727767-Polymerase Chain Reaction,
pubmed-meshheading:7727767-RNA, Messenger,
pubmed-meshheading:7727767-RNA Splicing,
pubmed-meshheading:7727767-Receptors, Interleukin-3,
pubmed-meshheading:7727767-Sequence Deletion
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene.
|
| pubmed:affiliation |
Department of Cell Biology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|