Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1995-5-19
pubmed:abstractText
The chemical structure of lipid A isolated from Porphyromonas gingivalis lipopolysaccharide was elucidated by compositional analysis, mass spectrometry, and nuclear magnetic resonance spectroscopy. The hydrophilic backbone of free lipid A was found to consisted of beta(1,6)-linked D-glucosamine disaccharide 1-phosphate. (R)-3-Hydroxy-15-methylhexadecanoic acid and (R)-3-hydroxyhexadecanoic acid are attached at positions 2 and 3 of the reducing terminal residue, respectively, and positions 2' and 3' of the nonreducing terminal unit are acylated with (R)-3-O-(hexadecanoyl)-15-methylhexadecanoic acid and (R)-3-hydroxy-13-methyltetradecanoic acid, respectively. The hydroxyl group at position 4' is partially replaced by another phosphate group, and the hydroxyl groups at positions 4 and 6' are unsubstituted. Considerable heterogeneity in the fatty acid chain length and the degree of acylation and phosphorylation was detected by liquid secondary ion-mass spectrometry (LSI-MS). A significant pseudomolecular ion of lipid A at m/z 1,769.6 [M-H]- corresponding to a diphosphorylated GlcN backbone bearing five acyl groups described above was detected in the negative mode of LSI-MS. Predominant ions, however, were observed at m/z 1,434.9 [M-H]- and m/z 1,449.0 [M-H]-, each representing monophosphoryl lipid A lacking (R)-3-hydroxyhexadecanoic and (R)-3-hydroxy-13-methyltetradecanoic acids, respectively. The presence of mono- and diphosphorylated lipid A species was also confirmed by LSI-MS of de-O-acylated lipid A (m/z 955.3 and 1,035.2, respectively).
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-13152076, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-13835291, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-14135466, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-1668251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-1691266, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-1702762, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-1730218, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-1904818, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-196857, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-19922, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2007601, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2040305, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-24002, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-24073, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2509563, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2600585, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2640312, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2759091, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2846816, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-2933084, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-3151207, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-3268745, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-3546137, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-3882561, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-3988753, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-4029152, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-4448988, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-452765, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-5069711, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-6222173, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-6362631, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-6583243, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-6653558, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-6749846, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-6980191, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-7084225, http://linkedlifedata.com/resource/pubmed/commentcorrection/7721702-770165
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2098-106
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Structural study on the free lipid A isolated from lipopolysaccharide of Porphyromonas gingivalis.
pubmed:affiliation
Department of Oral Microbiology, Kanagawa Dental College, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't