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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1995-5-10
|
| pubmed:abstractText |
The construction and operation of a cell for pressure- and temperature-controlled direct electrochemical studies of oxygen-sensitive biological materials is described. The stainless steel electrochemical cell, which contains a pyrolytic graphite working electrode, and Ag/AgCl counter and reference electrodes in an isolated compartment, easily maintains pressures between 1 and 2000 atm, and temperatures between 4 degrees C and at least 100 degrees C. The utility of the cell was demonstrated by determining the reduction potential of a redox dye, phenol red, by square wave voltammetry as a function of pressure and temperature. Thermodynamic parameters associated with electron transfer (delta V degree, delta beta, delta S degree, and delta H degree) were determined from the pressure and temperature/reduction potential profiles. Both pressure- and temperature-dependent reduction potentials of phenol red were attributed to differences in solvent interactions between its oxidation states. A discussion regarding the analysis of electrostatic interaction energies of biomolecules through pressure- and temperature-controlled electrochemical studies is presented.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
224
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
180-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1995
|
| pubmed:articleTitle |
A pressure- and temperature-controlled electrochemical cell for investigating biological electron transfer reactions.
|
| pubmed:affiliation |
Department of Chemistry, Florida Institute of Technology, Melbourne 32901-6988.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|