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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1995-5-4
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pubmed:abstractText |
The hippocampal nicotinic acetylcholine receptor (nAChR) is a newly identified ligand-gated ion channel that is blocked by the snake toxin alpha-bungarotoxin (alpha-BGT) and that probably contains the alpha 7 nAChR subunit in its structure. Here its ion selectivity was characterized and compared with that of the N-methyl-D-aspartate (NMDA) receptor channel. The reversal potentials (VR) of acetylcholine- and NMDA-activated whole-cell currents were determined under various ionic conditions. Using ion activities and a Goldman-Hodgkin-Katz equation for VR shifts in the presence of Ca2+, permeability ratios were calculated. For the alpha-BGT-sensitive nAChR, PNa/PCs was close to 1 and Cl- did not contribute to the currents. Changing the [Ca2+]0 from 1 to 10 mM, the VRs of the nAChR and NMDA currents were shifted by +5.6 +/- 0.4 and +8.3 +/- 0.4 mV, respectively, and the nAChR current decay was accelerated. These shifts yielded PCa/PCss of 6.1 +/- 0.5 for the nAChR channel and 10.3 +/- 0.7 for the NMDA channel. Thus, the neuronal alpha-BGT-sensitive nAChR is a cation channel considerably selective to Ca2+ and may mediate a fast rise in intracellular Ca2+ that would increase in magnitude with membrane hyperpolarization.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1285313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1310863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1370370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1375319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1569927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1586487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1697342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1702646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1708819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1710438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1716680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1722135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1753378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-18128147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-1978344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-201850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2054187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2203097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2259453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2280895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2369519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2438642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2451020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2457089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2558167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2663167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-2845577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-312319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-5713452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-6247423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-626914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-7507338,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-7512242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-7514645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-7525930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-7678857,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-7688468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-8247271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-8301367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-8312496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7696505-8510022
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
516-24
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:7696505-Acetylcholine,
pubmed-meshheading:7696505-Animals,
pubmed-meshheading:7696505-Bungarotoxins,
pubmed-meshheading:7696505-Calcium,
pubmed-meshheading:7696505-Cell Membrane Permeability,
pubmed-meshheading:7696505-Cesium,
pubmed-meshheading:7696505-Chlorides,
pubmed-meshheading:7696505-Hippocampus,
pubmed-meshheading:7696505-Ion Channel Gating,
pubmed-meshheading:7696505-Membrane Potentials,
pubmed-meshheading:7696505-Patch-Clamp Techniques,
pubmed-meshheading:7696505-Rats,
pubmed-meshheading:7696505-Rats, Sprague-Dawley,
pubmed-meshheading:7696505-Receptors, Nicotinic
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pubmed:year |
1995
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pubmed:articleTitle |
alpha-Bungarotoxin-sensitive hippocampal nicotinic receptor channel has a high calcium permeability.
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pubmed:affiliation |
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore 21201-1559.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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