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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1993-12-9
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pubmed:abstractText |
In this study we report on the developmental and regional expression of two brain-specific isoforms of tropomyosin, TMBr-1 and TMBr-3, that are generated from the rat alpha-tropomyosin gene via the use of alternative promoters and alternative RNA splicing. Western blot analysis using an exon-specific peptide polyclonal antibody revealed that the two isoforms are differentially expressed in development with TMBr-3 appearing in the embryonic brain at 16 days of gestation, followed by the expression of TMBr-1 at 20 days after birth. TMBr-3 was detected in all brain regions examined, whereas TMBr-1 was detected predominantly in brain areas that derived from the prosencephalon. Immunocytochemical studies on mixed primary cultures made from rat embryonic midbrain indicate that expression of the brain-specific epitope is restricted to neurons. The developmental pattern and neuronal localization of these forms of tropomyosin suggest that these isoforms have a specialized role in the development and plasticity of the nervous system.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1408826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1411520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1585177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1685706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1689409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1796905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1821676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1825520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1839162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-1839712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-2001841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-2054646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-2320008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-2384759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-2943217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-3052284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-3549996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-379013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-3924660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-6083453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-6203917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-6283379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-6447472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-6756641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-6809764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-69656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-8416988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7694294-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9857-61
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7694294-Aging,
pubmed-meshheading:7694294-Amino Acid Sequence,
pubmed-meshheading:7694294-Animals,
pubmed-meshheading:7694294-Antibodies,
pubmed-meshheading:7694294-Base Sequence,
pubmed-meshheading:7694294-Brain,
pubmed-meshheading:7694294-DNA Primers,
pubmed-meshheading:7694294-Embryonic and Fetal Development,
pubmed-meshheading:7694294-Epitopes,
pubmed-meshheading:7694294-Exons,
pubmed-meshheading:7694294-Gene Expression Regulation,
pubmed-meshheading:7694294-Gestational Age,
pubmed-meshheading:7694294-Immunoblotting,
pubmed-meshheading:7694294-Kidney,
pubmed-meshheading:7694294-Liver,
pubmed-meshheading:7694294-Molecular Sequence Data,
pubmed-meshheading:7694294-Muscles,
pubmed-meshheading:7694294-Neurons,
pubmed-meshheading:7694294-Organ Specificity,
pubmed-meshheading:7694294-Peptides,
pubmed-meshheading:7694294-Polymerase Chain Reaction,
pubmed-meshheading:7694294-Rats,
pubmed-meshheading:7694294-Rats, Sprague-Dawley,
pubmed-meshheading:7694294-Tropomyosin
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pubmed:year |
1993
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pubmed:articleTitle |
Brain-specific tropomyosins TMBr-1 and TMBr-3 have distinct patterns of expression during development and in adult brain.
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pubmed:affiliation |
Cold Spring Harbor Laboratory, NY 11724.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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