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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1993-9-28
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pubmed:abstractText |
Isolated smooth muscle cells from the circular layer of pig and guinea-pig ileum were used to study the effect of prostaglandin E2 (PGE2) and three PGE2 receptor (EP) agonists; iloprost (EP1), butaprost (EP2) and enprostil (EP3). In pig cells, PGE2 and enprostil induced cell contraction (22.1 and 21.5% shortening of cell length, obtained at 10 nM for PGE2 and 1 nM for enprostil, respectively). Iloprost and butaprost had no contractile effect. However, the cholecystokinin octapeptide (CCK-8; 10 nM)-induced contraction was inhibited when cells were preincubated with iloprost or butaprost. In guinea-pig cells, PGE2, butaprost and iloprost induced cell contraction, whereas enprostil had no effect (23.1% for 10 nM PGE2, 22.8% for 1 nM butaprost and 22.6% for 10 nM iloprost). Preincubation with SC19220 (EP1 antagonist) inhibited the PGE2-, butaprost- and iloprost-induced contractions. When the contractile effect of PGE2, butaprost and iloprost was inhibited by addition of SC19220, these agents inhibited the cell contraction induced by CCK-8 (1 nM). Smooth muscle cells from guinea-pig and pig ileum express two PGE2 receptor subtypes that induce opposite effect. EP1 and EP3 receptors mediate cell contraction in guinea-pig and pig, respectively, whereas EP2 receptors mediate cell relaxation in both species.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alprostadil,
http://linkedlifedata.com/resource/pubmed/chemical/Dibenz(b,f)(1,4)oxazepine-10(11H)-ca...,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Enprostil,
http://linkedlifedata.com/resource/pubmed/chemical/Iloprost,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandins E, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin,
http://linkedlifedata.com/resource/pubmed/chemical/Sincalide,
http://linkedlifedata.com/resource/pubmed/chemical/butaprost
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0014-2999
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
237
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7689467-Alprostadil,
pubmed-meshheading:7689467-Animals,
pubmed-meshheading:7689467-Dibenz(b,f)(1,4)oxazepine-10(11H)-carboxylic acid...,
pubmed-meshheading:7689467-Dinoprostone,
pubmed-meshheading:7689467-Enprostil,
pubmed-meshheading:7689467-Guinea Pigs,
pubmed-meshheading:7689467-Ileum,
pubmed-meshheading:7689467-Iloprost,
pubmed-meshheading:7689467-Male,
pubmed-meshheading:7689467-Muscle, Smooth,
pubmed-meshheading:7689467-Muscle Contraction,
pubmed-meshheading:7689467-Prostaglandins E, Synthetic,
pubmed-meshheading:7689467-Receptors, Prostaglandin,
pubmed-meshheading:7689467-Sincalide,
pubmed-meshheading:7689467-Swine
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pubmed:year |
1993
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pubmed:articleTitle |
Stimulatory (EP1 and EP3) and inhibitory (EP2) prostaglandin E2 receptors in isolated ileal smooth muscle cells.
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pubmed:affiliation |
Department of Pharmacology, INRA, Toulouse, France.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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