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rdf:type |
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lifeskim:mentions |
umls-concept:C0001511,
umls-concept:C0026473,
umls-concept:C0054942,
umls-concept:C0064830,
umls-concept:C0078056,
umls-concept:C0086418,
umls-concept:C0205217,
umls-concept:C0209606,
umls-concept:C0225336,
umls-concept:C0348013,
umls-concept:C0441712,
umls-concept:C0851827,
umls-concept:C1701901
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pubmed:issue |
2
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pubmed:dateCreated |
1993-9-10
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pubmed:abstractText |
Expression of adhesion molecules on endothelial cells (EC) can be up-regulated or induced by cytokines. The aim of the present study was to investigate the effect of IL-4 on both the expression of adhesion molecules on EC and monocyte adhesion to EC. Flow cytometric analysis showed that VCAM-1 expression on EC was up-regulated after stimulation with IL-4 for 24 h, whereas the expression of E-selectin (formerly called endothelial leucocyte adhesion molecule-1 (ELAM-1)) was not enhanced, and that of intercellular adhesion molecule-1 (ICAM-1) only slightly. The adhesion of monocytes to EC increased to maximum values upon stimulation of EC with IL-4 for 24 h. Coating of monocytes with MoAb against the integrin beta 2-subunit (CD18) significantly inhibited their adhesion to IL-4-stimulated EC; maximal inhibition was found when monocytes were coated with anti-CD18 MoAb in combination with MoAb against CD49d (the alpha-chain of VLA-4), whereas no inhibition was found when monocytes were coated only with MoAb against CD49d. Monocyte adhesion was not significantly inhibited when IL-4-stimulated EC were coated with MoAbs against ICAM-1 or VCAM-1 alone or in combination. Adhesion of monocytes was inhibited to a greater extent when in addition to coating of monocytes with MoAb against CD18 the EC were coated with MoAb against VCAM-1. From these results we conclude that monocytes bind to IL-4-stimulated EC via interaction of CD11/CD18 molecules on the monocytes with an as yet unknown endothelial ligand, and interaction of VLA-4 on monocytes with VCAM-1 on EC.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1378077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1560199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1647238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1675131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1689464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1693625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1695647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1697486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1698865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1701391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1702807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1709380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1709678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1710835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1711540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1717365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1719086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1723715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1968349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1969883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1972164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1973184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1974032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1980124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1990522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-1997656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2188667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2219269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2462353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2497351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2538512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2564407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2688898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2827173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2896958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-293671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-2970644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-3068126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-3134364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-3297106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-3525675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-3877078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-4355998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-6199797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-6225125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7688678-6435125
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD11,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Very Late Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
292-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7688678-Humans,
pubmed-meshheading:7688678-Peroxidase,
pubmed-meshheading:7688678-Monocytes,
pubmed-meshheading:7688678-Endothelium, Vascular,
pubmed-meshheading:7688678-Cell Adhesion,
pubmed-meshheading:7688678-Antigens, CD,
pubmed-meshheading:7688678-Cell Adhesion Molecules,
pubmed-meshheading:7688678-Interleukin-4,
pubmed-meshheading:7688678-Antigens, CD18,
pubmed-meshheading:7688678-Receptors, Very Late Antigen,
pubmed-meshheading:7688678-Antigens, CD11
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