Linked Life Data

7686261

Source:http://linkedlifedata.com/resource/pubmed/id/7686261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-7-26
pubmed:abstractText
This report describes a reproducible, straightforward approach to sequencing double-stranded DNA products from the polymerase chain reaction (PCR) for analysis of mutations. The sequencing protocol is a modification of that published by Kretz (Kretz et al., 1989) and has been successful in the hands of a number of investigators working on diverse projects. Following this procedure, PCR DNA products generated from bacterial sources (including pBR322 and F' derivatives), as well as cDNA and genomic DNA from both hamster and human cell lines, have been sequenced with equal success. Close attention to the molar ratio of nucleotides to double-stranded DNA template present during the labeling reaction ensures best results.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-5
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Sequencing of double-stranded polymerase chain reaction products for mutation analysis.
pubmed:affiliation
Case Western Reserve University, Ireland Cancer Center, Cleveland, OH.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't