Linked Life Data

7682072

Source:http://linkedlifedata.com/resource/pubmed/id/7682072

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-5-3
pubmed:abstractText
Polymerase chain reaction amplification of reverse transcribed RNA (RNA-PCR) was used to isolate overlapping cDNA clones encoding an inducible form of nitric oxide synthase (NOS) from purified rat hepatocytes. NOS gene expression in hepatocytes is dependent upon bacterial lipopolysaccharide (LPS) and cytokine treatment. The predicted amino acid sequence of rat hepatocyte inducible NOS is 94% identical with that from the mouse macrophage cell line, RAW264.7. Differences between the two sequences occur as sporadic amino acid substitutions with no major gaps or insertions. These differences are most likely the result of species variability which suggests that hepatocytes and macrophages express the same NOS gene upon induction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
191
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
767-74
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Hepatocytes and macrophages express an identical cytokine inducible nitric oxide synthase gene.
pubmed:affiliation
Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, North Carolina 27709.
pubmed:publicationType
Journal Article, Comparative Study