Linked Life Data

7680533

Source:http://linkedlifedata.com/resource/pubmed/id/7680533

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 2
pubmed:dateCreated
1993-4-6
pubmed:abstractText
Peritubular protein concentration affects the rate of volume absorption by the proximal tubule, and this effect may be mediated across the tubular basement membrane. To examine the permeability properties of this membrane, segments of mouse proximal straight tubule were isolated and perfused, and cells were removed using deoxycholate. Volume loss from the isolated perfused basement membrane was measured using blue dextran as a volume marker. Each segment was exposed alternately to solutions containing either no colloid or 6% bovine serum albumin. With rates of perfusion averaging 15.9 +/- 1.3 nl/min, rates of volume loss averaged 0.7 +/- 1.7 with no protein in the bath but were significantly higher with albumin, 16.5 +/- 2.1 nl.min-1.mm-1 (n = 14; mean length = 0.4 +/- 0.04 mm). Significant volume flux was also seen when the bath was changed to contain dextran [weight-averaged mol wt (Mw) approximately 70,000 or approximately 500,000]. In separate experiments, the net flux of albumin with albumin in the bath averaged 4.2 +/- 1.2 pmol.min-1.mm-1 (n = 5). The results show that this basement membrane has a high water permeability and significantly restricts the movement of albumin. Permeabilities for osmotic water flow and albumin were estimated from modeling fluxes in individual segments and found to average 70.6 +/- 9.8 cm/s (sigma = 0.1; 11.4 +/- 1.6 for sigma = 1) and 1.6 +/- 0.3 micron/s (assuming sigma = 0.1; 0.8 +/- 0.2 for sigma = 1), respectively. Using these permeabilities, it is demonstrated that interstitial protein may significantly reduce hydrostatic pressure in the intercellular space by oncotic action across the basement membrane.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F328-36
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Oncotic effects across isolated perfused renal tubular basement membrane.
pubmed:affiliation
Department of Anatomy, Indiana University School of Medicine, Indianapolis 46202-5120.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't