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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1993-4-6
|
| pubmed:abstractText |
To investigate the functional change of stromal cells along with differentiation, we used a differentiation-inducible mouse embryo fibroblast cell line, C3H10T1/2 (10T1/2). Stably determined preadipocyte and myoblast cell lines were established after a brief exposure of 10T1/2 cells to 5-azacytidine. These cell lines terminally differentiated into adipocytes and myotubes, respectively, under appropriate conditions. The hematopoiesis-supporting ability of each 10T1/2-derived cell line was examined by coculture with FACS-sorted murine hematopoietic stem cells (Thy-1lo c-kit+ Lin-). The number of granulocyte-macrophage progenitors (CFU-GM) was slightly reduced after 7 days of culture with parent 10T1/2 fibroblasts, whereas a marked increase in CFU-GM number was observed when the cells were cultured on preadipocytes. Mature adipocytes and myogenically determined cell lines, on the other hand, did not support CFU-GM growth. Further, Northern analysis showed that the preadipocyte cell line acquired the ability to produce a significant amount of stem cell factor (SCF), interleukin-6 (IL-6), leukemia inhibitory factor, and macrophage colony-stimulating factor mRNAs in response to IL-1 or lipopolysaccharide stimulation. Terminal adipocytic differentiation resulted in reduced ability to express these cytokine mRNAs. Similarly, highest IL-6 activity was detected in the supernatant of preadipocyte culture, whereas adipocytes did not secrete IL-6 even after IL-1 stimulation. Interestingly, hematopoiesis-nonsupporting myoblasts and myotubes also expressed abundant SCF mRNA, suggesting that SCF, per se, may not be sufficient for stem cell growth and survival. The 10T1/2-derived cell lines could provide a valuable tool to aid in the analysis of stromal cell development and the search for novel stromal factors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
81
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1184-92
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7680242-Adipose Tissue,
pubmed-meshheading:7680242-Animals,
pubmed-meshheading:7680242-Base Sequence,
pubmed-meshheading:7680242-Cell Differentiation,
pubmed-meshheading:7680242-Cell Line,
pubmed-meshheading:7680242-Cytokines,
pubmed-meshheading:7680242-Embryo, Mammalian,
pubmed-meshheading:7680242-Extracellular Matrix,
pubmed-meshheading:7680242-Female,
pubmed-meshheading:7680242-Fibroblasts,
pubmed-meshheading:7680242-Hematopoiesis,
pubmed-meshheading:7680242-Hematopoietic Cell Growth Factors,
pubmed-meshheading:7680242-Mice,
pubmed-meshheading:7680242-Mice, Inbred C3H,
pubmed-meshheading:7680242-Mice, Inbred C57BL,
pubmed-meshheading:7680242-Molecular Sequence Data,
pubmed-meshheading:7680242-Muscles,
pubmed-meshheading:7680242-Pregnancy,
pubmed-meshheading:7680242-RNA, Messenger,
pubmed-meshheading:7680242-Stem Cell Factor,
pubmed-meshheading:7680242-Stromal Cells
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Changes in hematopoiesis-supporting ability of C3H10T1/2 mouse embryo fibroblasts during differentiation.
|
| pubmed:affiliation |
Department of Hematology-Oncology, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|