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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1995-10-12
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pubmed:abstractText |
An EPR "spectroscopic ruler" was developed using a series of alpha-helical polypeptides, each modified with two nitroxide spin labels. The EPR line broadening due to electron-electron dipolar interactions in the frozen state was determined using the Fourier deconvolution method. These dipolar spectra were then used to estimate the distances between the two nitroxides separated by 8-25 A. Results agreed well with a simple alpha-helical model. The standard deviation from the model system was 0.9 A in the range of 8-25 A. This technique is applicable to complex systems such as membrane receptors and channels, which are difficult to access with high-resolution NMR or x-ray crystallography, and is expected to be particularly useful for systems for which optical methods are hampered by the presence of light-interfering membranes or chromophores.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1312255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1328890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1591245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1660187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-1661030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-2160734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-262414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-2748584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-345054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-5233469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-789892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8218270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8218281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8382373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8428572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8469972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7667275-8493553
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8239-43
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1995
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pubmed:articleTitle |
Determination of the distance between two spin labels attached to a macromolecule.
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pubmed:affiliation |
Department of Chemistry, Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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