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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0017337,
umls-concept:C0017428,
umls-concept:C0033634,
umls-concept:C0033684,
umls-concept:C0085862,
umls-concept:C0162326,
umls-concept:C0162610,
umls-concept:C0205197,
umls-concept:C0332120,
umls-concept:C1299583,
umls-concept:C1549571,
umls-concept:C1608386,
umls-concept:C2348205,
umls-concept:C2700640
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pubmed:issue |
4
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pubmed:dateCreated |
1995-10-2
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pubmed:databankReference |
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pubmed:abstractText |
The gene tpa-1 on chromosome IV of the nematode Caenorhabditis elegans plays a major and definitive role in the adversary action of tumour-promoting phorbol esters, which induce growth arrest and locomotory distress in the animal. The gene was deduced to code for a protein kinase C (PKC) homologue by molecular cloning. We have now sequenced the complete genomic and complementary DNAs for tpa-1 and have analysed their structural features in detail: (1) tpa-1 spans over 20 kb consisting of eleven exons and ten introns; (2) two different-sized mRNAs are generated from the tpa-1 locus; (3) both mRNAs are trans-spliced to the trans-spliced leader SL1; (4) both mRNAs encode PKC isoforms, which are most similar to Ca(2+)-independent novel PKC0; (5) the two PKC isoforms differ from each other in that the smaller lacks the amino-terminal region of the larger corresponding to the first four exons and a portion of the fifth exon; and (6) three introns are located at; identical positions in the polypeptide sequences aligned between the C. elegans tpa-1 product and a PKC of the fruit fly Drosophila melanogaster.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-2836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
251
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pubmed:geneSymbol |
tpa-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
477-85
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7658466-Amino Acid Sequence,
pubmed-meshheading:7658466-Animals,
pubmed-meshheading:7658466-Base Sequence,
pubmed-meshheading:7658466-Caenorhabditis elegans,
pubmed-meshheading:7658466-Caenorhabditis elegans Proteins,
pubmed-meshheading:7658466-DNA, Complementary,
pubmed-meshheading:7658466-DNA, Helminth,
pubmed-meshheading:7658466-Drosophila melanogaster,
pubmed-meshheading:7658466-Exons,
pubmed-meshheading:7658466-Genes, Helminth,
pubmed-meshheading:7658466-Introns,
pubmed-meshheading:7658466-Molecular Sequence Data,
pubmed-meshheading:7658466-Protein Kinase C,
pubmed-meshheading:7658466-Protein-Tyrosine Kinases,
pubmed-meshheading:7658466-RNA, Helminth,
pubmed-meshheading:7658466-RNA, Messenger,
pubmed-meshheading:7658466-RNA Splicing,
pubmed-meshheading:7658466-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:7658466-Restriction Mapping,
pubmed-meshheading:7658466-Sequence Analysis, DNA,
pubmed-meshheading:7658466-Sequence Homology, Amino Acid
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pubmed:year |
1995
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pubmed:articleTitle |
The tpa-1 gene of Caenorhabditis elegans encodes two proteins similar to Ca(2+)-independent protein kinase Cs: evidence by complete genomic and complementary DNA sequences of the tpa-1 gene.
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pubmed:affiliation |
Fundamental Research Laboratories, NEC Corporation, Tsukuba, Japan.
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pubmed:publicationType |
Journal Article
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