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rdf:type |
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lifeskim:mentions |
umls-concept:C0003261,
umls-concept:C0003320,
umls-concept:C0022702,
umls-concept:C0024264,
umls-concept:C0032594,
umls-concept:C0034493,
umls-concept:C0038402,
umls-concept:C0456389,
umls-concept:C0871261,
umls-concept:C0936012,
umls-concept:C1257890,
umls-concept:C1522642,
umls-concept:C1533691,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
2
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pubmed:dateCreated |
1976-4-15
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pubmed:abstractText |
Peripheral blood lymphocytes (PBL) of rabbits previously hyperimmunized against streptococcal groups A and A-variant antigens were stimulated in vitro by the corresponding vaccines to produce group-specific antibody. This response was dependent on an optimal cell density (2 X 10(6) cells/ml), on the presence of antigen, it was specific and cross-reactive due to a shared rhamnose backbone of the two polysaccharide antigens, and it was highly selective, such that in a 42-55-day culture 1 out of 20 viable cells was a specific PFC. During the exponential increase of the antibody concentration at a constant number of PFC, antibodies were secreted at a rate of 2.4 X 10(4) molecules/s per cell until a plateau level of antibody (40 mug/culture) was reached. The microculture system was used to determine the minimal frequency of group polysaccharide-specific precursor cells in the blood. Independent of the time elapsed since the last immunization this frequency was 1-3 X 10(-5), i.e., in the range of 1-2.8 X 10(2) precursor cells per ml blood. This number was further used together with the clonotype analysis of the culture supernates to calculate the frequencies of precursors of major and minor clonotypes. A hierachy of persisting clonal memory precursor cells was found indicating that clonal dominance is determined by locked-in frequency patterns and therefore it is a phenomenon based on numbers of cells that respond to the antigen.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-1135609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-13367334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-14097346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-14316952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4098533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4133618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4140053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4543318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4561843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4563349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4576501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4587738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4590818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4606658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4609776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4727916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-4941770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/765425-6034749
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
360-71
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:765425-Animals,
pubmed-meshheading:765425-Antibody Formation,
pubmed-meshheading:765425-Clone Cells,
pubmed-meshheading:765425-Immune Sera,
pubmed-meshheading:765425-Immunization, Secondary,
pubmed-meshheading:765425-Immunoglobulin G,
pubmed-meshheading:765425-Immunologic Memory,
pubmed-meshheading:765425-Kinetics,
pubmed-meshheading:765425-Lymphocytes,
pubmed-meshheading:765425-Polysaccharides, Bacterial,
pubmed-meshheading:765425-Rabbits,
pubmed-meshheading:765425-Streptococcus pyogenes
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pubmed:year |
1976
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pubmed:articleTitle |
Antibody response of rabbit blood lymphocytes in vitro. Kinetics, clone size, and clonotype analysis in response to streptococcal group polysaccharide antigens.
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pubmed:publicationType |
Journal Article
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