Linked Life Data

7645542

Source:http://linkedlifedata.com/resource/pubmed/id/7645542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-9-19
pubmed:abstractText
Studies were undertaken to investigate the hypothesis that infiltrating glomerular macrophages in experimental glomerulonephritis are activated to produce oxygen-free radicals that are capable of enhancing oxidation of low-density lipoprotein (LDL). Low-density lipoprotein oxidation was assessed by increased electrophoretic mobility on agarose gel electrophoresis and by the generation of thiobarbituric acid-reactive substances. Lipoprotein uptake, degradation, and re-esterification by macrophages were assessed by measuring 14C-oleic acid incorporation into cholesteryl oleate. Both peritoneal and glomerular macrophages have the ability to oxidize LDL to a form showing increased mobility on agarose gel electrophoresis. However, LDL incubated with glomerular macrophages underwent greater oxidation, resulting in increased generation of thiobarbituric acid-reactive substances (15.1 +/- 1.2 nmol malondialdehyde/mg LDL protein v 7.2 +/- 2.1 nmol malondialdehyde/mg LDL protein; P < 0.01). In addition, glomerular macrophages modified LDL to a form that greatly enhanced cellular synthesis of cholesteryl oleate compared with peritoneal macrophage-modified LDL (30 +/- 11 pmol/10(6) cells/hr v 10 +/- 4 pmol/10(6) cells/hr; P < 0.01). Superoxide dismutase, a scavenger of superoxide anion, inhibited macrophage-mediated oxidation of LDL. These results suggest that glomerular macrophages from nephritic rats are activated to modify LDL to a form avidly taken up by macrophage scavenger receptors. Thus, enhanced formation of oxidized LDL by infiltrating glomerular macrophages may contribute to glomerular injury in nephrotoxic serum nephritis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0272-6386
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
362-7
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Glomerular macrophages in nephrotoxic serum nephritis are activated to oxidize low-density lipoprotein.
pubmed:affiliation
Department of Medicine, Montefiore Medical Center, Bronx, NY, USA.
pubmed:publicationType
Journal Article