Linked Life Data

7645425

Source:http://linkedlifedata.com/resource/pubmed/id/7645425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1995-9-21
pubmed:abstractText
In vivo bromodeoxyuridine (BrdUrd) labelling and bivariate BrdUrd/DNA analysis was used to evaluate cell cycle kinetics in a rat tumor model known to be sensitive to dietary fatty acid manipulation. Fish oil supplementation significantly reduced the rate of BrdUrd movement relative to DNA content, indicating prolongation of the DNA replication time. This finding, which accounted for most of the decrease in tumor growth rate in the fish oil-fed group, represents the first description of an alteration in S phase duration by an extrinsic factor. The significance of this finding is discussed in relation to current understanding of cell cycle regulation. Fish oil feeding is associated with slower growth rate in certain tumors (1,2). According to current concepts of cellular proliferation (3), regulation of growth by extrinsic factors is thought to precede the S phase. This statement is based on the notion that, within a given cell type, DNA replication time (S phase duration) is constant (4-6). Extensive evidence also supports an on/off mechanism of cell cycle regulation at the level of entry into the S phase (3). In this report, we present evidence showing, for the first time, that the S phase duration of fat-responsive tumor cells can be altered by dietary manipulation of fatty acids. Furthermore, these differences in S phase duration appear to account for all the in vivo variation in tumor growth resulting from fish oil feeding. Although the mechanism of this phenomenon remains unclear, our observations support increasing evidence for a regulatory step at the level of the nucleus. They are also important for understanding the relationship between dietary fat and tumor growth.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
375
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
149-56
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Fish oil and cell proliferation kinetics in a mammary carcinoma tumor model.
pubmed:affiliation
Clinical Nutrition Unit, Boston University Medical Center Hospital, Massachusetts 02118, USA.
pubmed:publicationType
Journal Article, Comparative Study