Linked Life Data

7636746

Source:http://linkedlifedata.com/resource/pubmed/id/7636746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1995-9-8
pubmed:abstractText
Distinct carbohydrates influenced the in vitro permeation of the somatostatin analogue octapeptide octreotide through Caco-2 cell monolayers. Apical addition of 20 mM D-glucose or D-xylose resulted in a 2.3- or 3.4-fold increased octreotide permeation, respectively. However, supplementation with 20 mM L-glucose or 20 mM D-fructose showed no permeation enhancement. Basolateral addition of D-glucose or D-xylose had no significant effect on octreotide permeation. Apical medium supplementation with D-glucose or D-xylose increased permeation of the extracellular marker [14C]polyethylene glycol 4000, indicating that both carbohydrates directly affected the paracellular route of octreotide absorption. Presence of 1 mM phlorizin decreased octreotide permeation through monolayers in the presence of glucose on average by 12.8%, suggesting that the Na(+)-dependent glucose cotransporter might be partially involved in the enhancement of the absorption process of octreotide. Octreotide was absorbed from ligated jejunal loops of rat small intestine with an absolute absorption efficiency of about 0.3%. Coadministration of D-glucose of D-xylose resulted in a 2.2- or 1.9-fold increased absorption of octreotide, whereas D-fructose showed no effect. When the peptide was given in the presence of glucose and 1 mM phlorizin, a significant reduction of absorption enhancement could be observed. Phlorizin did not inhibit octreotide absorption, when the peptide was given in the absence of glucose. The data suggest that in vivo the active transepithelial flux of solutes such as glucose contributes to the enhancement of peptide absorption.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-3565
pubmed:author
pubmed:issnType
Print
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
826-32
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Enteral absorption of octreotide: modulation of intestinal permeability by distinct carbohydrates.
pubmed:affiliation
Drug Delivery Systems, Sandoz Pharma Ltd., Basel, Switzerland.
pubmed:publicationType
Journal Article