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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-9-11
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pubmed:abstractText |
Superantigens are extremely potent activators of T lymphocytes. To develop a tumor-reactive superantigen for cancer therapy, we made a recombinant fusion protein of the superantigen staphylococcal enterotoxin A (SEA) and the Fab region of the C242 monoclonal antibody (C242Fab-SEA), which recognize human colon carcinoma cells. The therapeutic effect of C242Fab-SEA on colon-cancer growth was examined in lymphocyte-engrafted humanized SCID mice bearing intraperitoneally growing Colo205 colon carcinomas. i.v. injections of C242Fab-SEA significantly inhibited tumor growth. The anti-tumor effect required the presence of human T cells in the SCID mice. Optimal therapeutic effects were seen with C242Fab-SEA, but not with C242Fab fragment or SEA alone, demonstrating that both entities of the fusion protein were required. C242Fab-SEA-treated tumors showed a massive infiltration of T cells in the tumor parenchyme, and was accompanied by enhanced expression of ICAM-I and HLA-DR on the tumor cells. The results demonstrate that Fab-SEA fusion proteins convey superantigenicity on tumor cells which evoke T-cell-dependent suppression of tumor growth.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0020-7136
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
466-71
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:7635573-Animals,
pubmed-meshheading:7635573-Antibodies, Monoclonal,
pubmed-meshheading:7635573-Colonic Neoplasms,
pubmed-meshheading:7635573-Enterotoxins,
pubmed-meshheading:7635573-Female,
pubmed-meshheading:7635573-HLA-DR Antigens,
pubmed-meshheading:7635573-Humans,
pubmed-meshheading:7635573-Immunoglobulin Fab Fragments,
pubmed-meshheading:7635573-Intercellular Adhesion Molecule-1,
pubmed-meshheading:7635573-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:7635573-Mice,
pubmed-meshheading:7635573-Mice, SCID,
pubmed-meshheading:7635573-Neoplasm Transplantation,
pubmed-meshheading:7635573-Recombinant Fusion Proteins,
pubmed-meshheading:7635573-Superantigens,
pubmed-meshheading:7635573-T-Lymphocytes,
pubmed-meshheading:7635573-Transplantation, Heterologous,
pubmed-meshheading:7635573-Tumor Cells, Cultured,
pubmed-meshheading:7635573-Up-Regulation
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pubmed:year |
1995
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pubmed:articleTitle |
Tumor-reactive superantigens suppress tumor growth in humanized SCID mice.
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pubmed:affiliation |
Department of Tumor Immunology, Wallenberg Laboratory, University of Lund, Sweden.
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pubmed:publicationType |
Journal Article
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