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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1995-9-11
|
| pubmed:databankReference | |
| pubmed:abstractText |
Since a considerably high incidence of allelic loss on chromosome 2q was detected in lung carcinoma and a homozygous deletion at chromosome 2q33 was detected in a small cell lung carcinoma cell line, NCI-H82, a novel tumor suppressor gene has been suggested to be present in this chromosomal region. In the present study, we constructed a cosmid contig map covering the homozygous deleted region, which was estimated as being 220 kbp in size, and identified a gene from the deleted region. All of the coding exons of this gene were homozygously deleted in this cell line, while a 5'-non-coding exons was retained. Since the gene encodes a protein with striking similarity to several members of a family of phospholipase C, we designated this gene as PLC-L (phospholipase C-deleted in lung carcinoma). The PLC-L gene was expressed in a variety of fetal and adult organs including the lung. However, its expression was greatly reduced in seven of 13 (53.8%) of small cell lung carcinoma and 13 of 15 (86.7%) of non-small cell lung carcinoma cell lines. Since its homology to phospholipase C genes suggests the involvement of the PLC-L gene in inositol phospholipid-based intracellular signaling cascade, it is possible that aberrant expression of the PLC-L gene contributes to the genesis or progression of human lung carcinoma.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0964-6906
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:geneSymbol |
PLC-L
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
667-74
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7633416-Amino Acid Sequence,
pubmed-meshheading:7633416-Animals,
pubmed-meshheading:7633416-Base Sequence,
pubmed-meshheading:7633416-Carcinoma, Small Cell,
pubmed-meshheading:7633416-Chromosome Deletion,
pubmed-meshheading:7633416-Chromosome Mapping,
pubmed-meshheading:7633416-Chromosomes, Human, Pair 2,
pubmed-meshheading:7633416-Cosmids,
pubmed-meshheading:7633416-DNA, Complementary,
pubmed-meshheading:7633416-DNA Primers,
pubmed-meshheading:7633416-Homozygote,
pubmed-meshheading:7633416-Humans,
pubmed-meshheading:7633416-Lung Neoplasms,
pubmed-meshheading:7633416-Molecular Sequence Data,
pubmed-meshheading:7633416-Multigene Family,
pubmed-meshheading:7633416-Sequence Homology, Amino Acid,
pubmed-meshheading:7633416-Tumor Cells, Cultured,
pubmed-meshheading:7633416-Type C Phospholipases
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Identification of a novel phospholipase C family gene at chromosome 2q33 that is homozygously deleted in human small cell lung carcinoma.
|
| pubmed:affiliation |
National Cancer Center Research Institute, Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|