Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
16
|
pubmed:dateCreated |
1995-9-7
|
pubmed:abstractText |
Recently, the D13S25 locus, which is in close proximity to the retinoblastoma gene (RB-1) on chromosome band 13q14, was discussed to play a role in the pathogenesis of B-CLL. In the present study, we isolated two overlapping genomic DNA clones (termed c13S25) containing the D13S25 DNA segment and used them as probes to analyze 85 B-CLL cases by fluorescence in situ hybridization; of the 55 cases with two RB-1 copies, 13 exhibited hemizygous (n = 7) or homozygous (n = 6) deletion of D13S25. Of 29 cases with hemizygous deletion of RB-1, all but two also showed loss of D13S25 (hemizygous, n = 25; homozygous, n = 2). One case had a homozygous deletion of both loci. We conclude that deletion of D13S25 occurs in a substantial number of B-CLL without deletion of RB-1. However, in some cases there is deletion of RB-1 without loss of D13S25, suggesting that D13S25 is not the locus of the putative tumor suppressor gene. According to our data, such a gene is most likely located within the genomic region between D13S25 and RB-1.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0008-5472
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
55
|
pubmed:geneSymbol |
RB-1
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
3475-7
|
pubmed:dateRevised |
2007-11-15
|
pubmed:meshHeading |
pubmed-meshheading:7627948-Chromosome Deletion,
pubmed-meshheading:7627948-Chromosomes, Human, Pair 13,
pubmed-meshheading:7627948-DNA, Neoplasm,
pubmed-meshheading:7627948-Genes, Retinoblastoma,
pubmed-meshheading:7627948-Genetic Markers,
pubmed-meshheading:7627948-Heterozygote,
pubmed-meshheading:7627948-Homozygote,
pubmed-meshheading:7627948-Humans,
pubmed-meshheading:7627948-In Situ Hybridization, Fluorescence,
pubmed-meshheading:7627948-Leukemia, Lymphocytic, Chronic, B-Cell
|
pubmed:year |
1995
|
pubmed:articleTitle |
Heterogeneity of deletions involving RB-1 and the D13S25 locus in B-cell chronic lymphocytic leukemia revealed by fluorescence in situ hybridization.
|
pubmed:affiliation |
Medizinische Klinik und Poliklinik V. Universität Heidelberg, Germany.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|