Linked Life Data

7625768

Source:http://linkedlifedata.com/resource/pubmed/id/7625768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
1995-8-30
pubmed:databankReference
pubmed:abstractText
We have assembled a panel of monochromosomal somatic cell hybrids for use in gene mapping. DNA from each individual hybrid was used as a probe on normal human metaphases to identify the human chromosome and any fragments by reverse painting. To test the efficiency of the panel PCR amplification of DNA from the monochromosomal somatic cell hybrid panel was used in combination with human specific oligonucleotide primers to assign alpha-catenin (CTNNA1) and p21/WAF1 to chromosomes 5 and 6 respectively. These genes were localized further using hybrids containing specific translocations to 5q11-qter and 6p21 respectively. We also developed primers to enable us to assign 17 ESTs sequenced by the HGMP Resource Centre. The hybrid panel was developed with support of the UK HGMP and the DNA is available to all registered users.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-4800
pubmed:author
pubmed:issnType
Print
pubmed:volume
59
pubmed:geneSymbol
CTNNA1, WAF-1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
233-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Development of a panel of monochromosomal somatic cell hybrids for rapid gene mapping.
pubmed:affiliation
Human Genetic Resources, Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't